Refactoring gene clusters for yeast

Design protocol for yeast heterologous expression

Protocols: Fungal Project

DNA assembly by yeast homologous recombination

DNA assembly by Gibson assembly (also available on protocols.io)

QIAprep 96 Turbo miniprep for yeast plasmid DNA

Next-gen sequencing of assembled gene clusters (updated 01/18/17)

50:50 Method for genome modification in yeast

Barcoded deletion library screening

Barcode quantification using Affymetrix Genflex Tag 16K

Barcode quantification using a MiSeq benchtop sequencer

Quick yeast extraction for LC-MS (also available on protocols.io)

Laboratory protocols used by the Metabolite Chemistry Analysis Center, a ChEM-H Knowledge Center at Stanford University

Protocols: Plant Project

Reconstruction of plant biosynthetic pathway in Saccharomyces cerevisiae