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Protocols

Many of the network's protocols are published on the Genomes to Natural Products Network protocols.io group. This is done to provide open and permanent access via DOIs, authorship, version control, and commenting, in hopes of enabling collaboration and reproducibility.

Refactoring gene clusters for yeast

Design protocol for yeast heterologous expression

Protocols: Fungal Project

DNA assembly by yeast homologous recombination

DNA assembly by Gibson assembly (also available on protocols.io)

QIAprep 96 Turbo miniprep for yeast plasmid DNA

Yeast Colony PCR: it doesn't get any easier than this!

Library Preparation for Sequencing of Assembled Gene Clusters

50:50 Method for genome modification in yeast

Barcoded deletion library screening

Barcode quantification using Affymetrix Genflex Tag 16K

Barcode quantification using a MiSeq benchtop sequencer

Quick yeast organic extraction for LC-MS (also available on protocols.io)

Preparation of 1.5 mg/mL Sera-mag carbolylate modified magnetic particles

Magnetic particle based DNA purification

Laboratory protocols used by the Metabolite Chemistry Analysis Center, a ChEM-H Knowledge Center at Stanford University

Protocols: Plant Project

Reconstruction of plant biosynthetic pathway in Saccharomyces cerevisiae

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