Many of the network's protocols are published on the Genomes to Natural Products Network protocols.io group. This is done to provide open and permanent access via DOIs, authorship, version control, and commenting, in hopes of enabling collaboration and reproducibility.
Refactoring gene clusters for yeast
Protocols: Fungal Project
DNA assembly by yeast homologous recombination
DNA assembly by Gibson assembly (also available on protocols.io)
QIAprep 96 Turbo miniprep for yeast plasmid DNA
Yeast Colony PCR: it doesn't get any easier than this!
Library Preparation for Sequencing of Assembled Gene Clusters
50:50 Method for genome modification in yeast
Barcoded deletion library screening
Barcode quantification using Affymetrix Genflex Tag 16K
Barcode quantification using a MiSeq benchtop sequencer
Quick yeast organic extraction for LC-MS (also available on protocols.io)
Preparation of 1.5 mg/mL Sera-mag carbolylate modified magnetic particles
Protocols: Plant Project
Reconstruction of plant biosynthetic pathway in Saccharomyces cerevisiae
