pTARa is a BAC-based S. cerevisiae/E. coli/Streptomyces shuttle capture vector. This vector contains elements that allow pathways to be assembled in S. cerevisiae, characterized and maintained in E. coli, and conjugatively transferred into a wide range of Streptomycetes for heterologous expression studies.
Cloning large natural product gene clusters from the environment: piecing environmental DNA gene clusters back together with TAR.
Kim JH, Feng Z, Bauer JD, Kallifidas D, Calle PY, Brady SF.
Biopolymers. 2010 Sep;93(9):833-44. doi: 10.1002/bip.21450. PMID: 20577994